Characterization of a novel human papillomavirus DNA in the cervical carcinoma cell line ME180.
نویسندگان
چکیده
The human cervical carcinoma cell line ME180 was examined for human papillomavirus (HPV) DNA and RNA. The integrated DNA of a presumably new HPV type showing a relationship closer to HPV39 than to HPV18 was cloned and sequenced. HPV sequences from the E6-E7-E1 region are expressed as poly(A)+ RNAs.
منابع مشابه
APM-1, a novel human gene, identified by aberrant co-transcription with papillomavirus oncogenes in a cervical carcinoma cell line, encodes a BTB/POZ-zinc finger protein with growth inhibitory activity.
Integration of human papillomavirus (HPV) DNA into the host cell genome is an important step in cervical carcinogenesis. In tumour cells with integrated HPV DNA, transcription of viral oncogenes E6 and E7 continues into the flanking cellular sequences thereby producing viral-cellular fusion transcripts. Analysis of cellular sequences flanking the integrated HPV68 DNA in the cervical carcinoma c...
متن کاملDetection of Human Papillomavirus DNA by PCR in Esophageal Squamous Cell Carcinoma from Turkmen Sahra, North-East of Iran
Human papillomavirus (HPV) DNA has been identified in esophageal carcinomas. However, the incidence of HPV varies significantly in different geographical locations. In this study, neoplasms from Turkmen Sahra, a region in Golestan province in northeast part of Iran, with a high incidence of squamous cell carcinoma were analyzed for the presence of HPV DNA. Turkmen Sahra is located in the cancer...
متن کاملRelevant Allelic Frequency of Gene Polymorphism and Genetic Predisposition of Human Papillomavirus in Patients with Cervical Cancer
Backround: The present study investigated the correlation between p53 gene codon 72 polymorphism and 6 other genetic single nucleotide polymorphisms (SNPs) in patients with cervical cancer infected by HPV. Methods: 450 patients with cervical cancer (280 Squamous cell carcinoma and 170 Adenocarcinoma) were followed at hospitals in Iran from Dec. 2014 to Apr. 2015. Moreover, 100 age/sex-matche...
متن کاملThe effect of catechin metabolism derived from intestinal microbiota on Ca SKi cell proliferation
Background & aim: In Iran, cervical cancer is the second leading cause of death in women. Papillomavirus infection is the most important risk factor for the development of cervical cancer. Ca SKi cell is a human cervical carcinoma cell that binds the papillomavirus type 16 virus to its genome. Cactin is a type of phenol and a secondary metabolite of a plant. The purpose of this study was to inv...
متن کاملDetection and characterization of human papillomavirus type 45 DNA in the cervical carcinoma cell line MS751.
The cervical carcinoma-derived cell line MS751 was examined for human papillomavirus (HPV) DNA and RNA. A genomic fragment containing both viral and cellular sequences was cloned. Sequence analysis showed that MS751 cells contain a partially deleted HPV-45 genome integrated at a single chromosomal site. HPV sequences from the E6-E7 region are expressed as poly(A)+ RNA.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of virology
دوره 65 10 شماره
صفحات -
تاریخ انتشار 1991